Electrophoresis Reagents Sample Preparation
WSE-7010 EzLabel FluoroNeo
Fluorescent protein labeling kit for SDS-PAGE
EzLabel FluoroNeo is a kit for SDS sample preparation and simultaneously for fluorescent labeling of protein and polypeptide.
Purpose and Application Product Summary Features Specifications Documents Optional Accessories
A brief video guide below shows experimental flow from sample preparation, SDS-PAGE to fluorescent detection using EzLabel FluoroNeo.
WSE-7010 EzLabel FluoroNeo
Purpose and Application
☆ Visualization of electrophoresis pattern with stain FREE☆
Product Summary
Fluorescent labeling reaction completed in only 3 min
Amine group of protein is labeled in 3 min of reaction after mixing with EzLabel FluoroNeo.
EzLabel FluoroNeo is capable to use as a substitute for SDS-PAGE sample buffer and available for fluorescent protein labeling simultaneous with sample preparation.
Samples can be started to electrophoresis immediately after labeling reaction without column purification.
Immediate detection after electrophoresis
The electrophoresed pattern visualize immediately after electrophoresis without staining/destaining.
Protein bands emits 530 nm fluorescence by UV or Blue LED excitation light.
The excitation with BlueLED is enabled to detect proteins with high sensitivity as silver staining (observable the gel without removing gel plate holder).
Available for conventional experimental methods
Hardly to detect any differences in protein band mobility after labeling reaction.
The observed gel can proceed to western blotting as usual.
After blotting, transferred proteins on membrane can be detected by excitation light.
Features
✔ Immediate visualization protein bands after electrophoresis
Protein fluorescence is emitted by BlueLED or UV excitation light
[Ex: 330 (UV), 470 nm, Em: 530 nm]
✔ Stain FREE
Saving time
Unnecessary for liquid waste disporsal
✔ Hardly to detect any differences in protein band mobility
✔ Observable the gel with Blue LED without removing glass plate gel holder
✔ Observed gel is available for western blotting and protein fluorescence is kept after transfer on membrane
✔ Observed gel is available for CBB staining and silver staining
✔ Kit includes RIPA Lysis Buffer (free amine buffer), MW marker
Specifications
| Product Number | WSE-7010 |
|---|---|
| Product Name | EzLabel FluoroNeo |
| Compornents |
|
| Storage period | for 1 year at -20℃ |
| Package | 2000 samples (20μL of one sample) |
Experimantal flow of EzLabel FluoroNeo
Western blotting with EzLabel FluoroNeo
Lane 1: Non-labeled MW marker
Lane 2: Labeled MW marker
Lane 3: Non-labeled HepG2 extract protein
Lane 4: Labeled HepG2 extract protein
After blotting, transferred proteins on membrane can be detected by excitation light.
Continuously process of western blotting reaction can be proceeded.
Documents
Product Brochure
(0.80 MB)
Instruction Manual